Behaviour, Mazes, Tracking
Code: 47442/47443

Light/Dark Box and Open Field combination, for Rats or Mice Featured

Our Light/Dark cage allows to carry out the Light/Dark Conflict Test conveniently.

The Light/Dark Box is a characteristic tool used in the assessment of anxiety: the apparatus consists of a simple chamber divided into a dark and a light compartment. Rodents prefer darker areas over light areas: however when presented in a novel environment, rodents have a tendency to explore. These two conflicting emotions lead to observable anxiety-like symptoms.

Mice are allowed to move freely between the two chambers. The number of entries into the bright chamber, the duration of time spent there and the related exploratory behaviors, detected via a videotracking system, are reliable parameters for assessing anxiolytic effects that may be useful in identifying and/or screening of anxiolytic and anxiogenic agents.

The cage is available for Mice or Rats; standard model has transparent walls on the lit compartment, but an optional model with opaque white walls is also available. Our model consists of an external box and a black insert, thus making it convenient for use as an open field.

Light is not included

Light/Dark Box Light/Dark Box

Additional Info

    High-contrast colors Gives optimal results with any Video-Tracking software
    Non-reflecting surface Guarantees optimal camera view and no glare
    Options available Transparent or opaque white walls
    Reasonably rough “warm” surface texture Selected for best rodent comfort, non-slippery
    Quality materials Reliable and durable, easy to clean and store
    Physical 47443 Mouse 47442 Rat
    Dimensions 44x44cm 50x100cm
    Wall height 35cm 40cm
    Start Box (dark chamber) I.D. 42x20x35(h)cm 48x48x40(h)cm
    Test Box (light  chamber) I.D. 42x20x35(h)cm 48x48x40(h)cm
    External Wall color Transparent or opaque (white) Transparent or opaque (white)
    Weight 10Kg 26Kg
    Shipping Weight 14Kg 32Kg
    Packing Dimensions 55x55x27cm 105x105x20cm
    Warranty 47442/47443 is covered by a 24-month warranty

    The light/dark transition test was originally developed by Crawley and colleagues (Crawley and Goodwin, 1980) and subsequently validated by Costall et al (1989).

    The light/dark transition test is one of the most widely used tests to measure anxiety-like behavior in mice. The test is based on the innate aversion of rodents to brightly illuminated areas and on their spontaneous exploratory behavior in response to mild stressors, that is, novel environment and light. Time spent in the lit compartment, and the related exploratory behavior, are reliable parameters for assessing anxiolytic effects that may be useful in identifying and/or screening of anxiolytic and anxiogenic agents.

    The original apparatus consisted of a small dark safe compartment (one third) and a large illuminated aversive compartment (two thirds), while more recently other models have been validated (Takao and Miyakawa 2006) in which the size of the two chambers is the same. Another difference between the original design and other models available is that the lit compartment is made of opaque white plastic. These differences, namely the size and openness of the light chamber, allow for the simultaneous detection of bright-space anxiety as well as open-space anxiety in the original version of the test.

    47442 Light/Dark Box for Mice
    47443 Light/Dark Box for Rats
    47442-W Light/Dark Box for Mice, with white opaque walls
    47443-W Light/Dark Box for Rats, with white opaque walls
    60000 ANY-maze Software
    41700-040 B/W USB Camera, including day&night 2.7-13.5mm varifocal lens, 5m USB cable and ceiling support
    • J. Crawley and F. K. Goodwin: "Preliminary Report of a Simple Animal Behavior Model for the Anxiolytic Effects of Benzodiazepines" Pharmacology Biochemistry and Behavior 13(2): 167-170, 1980
    • B. Costall et alia: "The Effects of ACE Inhibitors Captopril and SQ29, 852 in Rodent Tests of Cognition" Pharmacology Biochemistry and Behavior 33(3): 573-579, 1989
    • M. Bourin and M. Hascoët: “The Mouse Light/Dark Box Test” J Vis Exp. 463(1): 55-65, 2003
    • K. Takao and T. Miyakawa: "Light/dark Transition Test for Mice" JoVE 1: e104-e104, 2006

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