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Animal
Mouse
Videotracking
Traditional, Infrared

Operant Fear Conditioning (OFC)

Product Code:49603

The Operant Fear Conditioning Cage couples a classic skinner box with sound and light cues for complex experiments, as well as standard operant tests and fear conditioning protocols.

Model
System component 2
Optional
This product warranty can be extended up to 24 Additional Months.
Discover more HERE!

Dual purpose systems to be used for Operant or Fear Conditioning experiments

A standard Fear Conditioning cage has been modified adding an operant wall with 1 pellet dispenser, 2 nose pokes, a house light and 2 cue lights above the nose pokes. The operant wall can be hidden by a removable plastic wall to use the cage as a standard Fear Conditioning.

Protocols, results and analyses are managed by Any-maze, through which virtually all Operant tests can be programmed:

  • Fixed Ratio
  • Variable Ratio
  • Progressive Ratio
  • Fixed Interval
  • Variable Interval
  • Extinction
  • Delayed Reinforcement and Discounting
  • Matching law
  • Reversal learning
  • Timeout

Also for Fear Conditioning the most common procedures and many more can be run via Any-maze:

  • Cued and Contextual
  • Trace
  • Extinction
  • Observational

 

Feature and benefits  
The same cage includes both reward and shock floor

Both Operant and Fear Conditioning experiments can be run in the same system

The isolation cubicle includes all electronics, shock and lights One isolation cubicle can be used for multiple types of experiments without any additional module
Multiple cages can be connected to 1 PC via USB High throughput experiments can be run
Procedures are created by a fully graphical interface No need for coding. Fast learning curve even for non IT-personnel
Operation
Read-Out USB to PC through AnyMaze, TTL I/O

General 

Shock

Constant current

Shock Intensity

from 0.1 to 3mA, in 0.1mA steps

Shock Duration

Duration: from 0.5 to 60 seconds

Cue Light

Dimmable visible 6500K

Pellet Dispenser

One with manger and reward detection

Nose Poke Two with detection and no odor delivery
Nose Poke LEDs Two, one yellow and one blue

Power 

100-240 VAC, 50/60 Hz, 60W max

Operating Temperature

10°C to 40°C; 5% to 95% RH (non-condensing)

Context

One set for Fear Conditioning tests
One set for operant experiments

Physical

Dimension Outer Size

Internal size with grid

17,5(d)x17,5(w)x16,5(h)cm

Internal size with floor 17,5(d)x17,5(w)x16,5(h)cm
External size (with Pellet Dispenser) 36,3(w)x20(d)x25,7(h)cm
Shipping dimensions

36x55x45xm

Weight

6Kg

Shipping Weight

10.5Kg

Warranty

Warranty Operant Fear Conditioning is covered by a 12-month warranty + 12 after product registration

 

The Operant Fear Conditioning system can be essentially used in three ways:

 

  1. As an Operant system: Operant experiments are fully programmable with the ANY-Maze software, and protocols ranging from Fixed or Variable Ratio, Fixed or Variable Schedule, Progressive Schedule, Delay Discounting, Extinction, etc.
  2. As a Fear Conditioning: The operant wall can be covered by a provided plastic wall, so that Fear Conditioning experiments can be performed in all their different declinations, taking advantage of the high accuracy of the Any-Maze freezing detection algorithm.
  3. As Avoidance and Conflict Tests (reward vs shock): The Operant Wall and the Fear Conditioning set-up can also be used in combination for Avoidance Experiments, for example by partially covering the grid floor with a small plastic platform and measuring the motivation of rodents to leave the platform and get the pellet, in spite of the shock risk (Bavo-Rivera et al., 2014, The Journal of Neuroscience).
Operant Fear Conditioning - What's in the box
49603 Operant Cage & Fear Conditioning cage with 2 nose pokes, minimum 2x2 coloured lights, white light (to be used as conditioning cue or omission cue), pellet dispenser with measuring feeder and electrified grid. Context and floor also available for free on request
49104 Pellet dispenser with interchangeable accessories for rat and mouse
Additional items (for Fear Conditioning System complete set-up)
46001

Fear-Conditioning Unit, including Isolation Cubicle 46000-596 (inside dimensions 39(d)x47(w)x49(h)cm), dual visible/I.R. light 46000-325, speaker 46000-165, controller on-board 46000-105, camera 47400-035, cables and power supply. System to be completed with video tracking software ANY-maze (full license or FC-version).

Software for Fear Conditioning System (the system requires ANY-maze version 7 or higher)
60000-FC ANY-maze software, FC-specific version (FEAR CONDITIONING TEST ONLY)
60000 ANY-maze software, full license (use for Fear Conditioning and all the other inplemented test)
Optionals
46000-101 Electric shock meter for Fear Conditioning system (essential instrument to mesure current between cages)
46002-D03 Rat Cage for Fear Conditioning, for thetered animal, with electrified grid floor, transparent walls, internal dimensions 25.5 width x 25.5 depth x 36 height cm. including 3 floors (gray, white and black) and 3 contexts (grey, stripes and checkerboard)
46003-D03 Mouse Cage for Fear Conditioning, for thetered animal, with electrified grid floor, transparent walls, internal dimensions 17 width x 17 depth x 25 height cm. including 3 plastic floors (gray, white and black) and 3 contexts (grey, stripes and checkerboard)
46004-D03 Mouse Cage XL for Fear Conditioning, for thetered animal, with electrified grid floor, transparent walls,  internal dimensions 25.5 width x 25.5 depth x 36 height cm. including 3 plastic floors (gray, white and black) and 3 contexts (grey, stripes and checkerboard)
46004-D01 Fear Conditioning Cage with frontal vertical door. Internal dimensions: 25.5(w)x26(d)x36(h) cm. Magnetic closure. Including 3 plastic floors (gray, white and black) and 3 contexts (grey, stripes and checkerboard
46005 Fear Conditioning Cage for Observational Learning, Internal dimension 35(w)x17(d)25(h)cm; (17x17 cm for each cage)

M. Jiang, S. Vanan, H. Tu, W. Zhang, Z. Zhang, S. Chia, S. E. Jang, X. Zeng, E. Yu, J. Xu, K. Guo, L. Zeng, 2020, "Amyloid precursor protein intracellular domain-dependent regulation of FOXO3a inhibits adult hippocampal neurogenesis", Neurobiology of aging

A. Sirri, V. Bianchi, M. Pelizzola, M.Mayhaus, P. Ricciardi-Castagnoli, D. Toniolo, P. D’Adamo, 2010, "Temporal gene expression profile of the hippocampus following trace fear conditioning", Brain Research

C.L. Bender, A. Otamendi, G.D. Calfa, V.A. Molina, 2018, “Prior Stress Promotes The Generalization Of Contextual Fear Memories: Involvement Of The Gabaergic Signaling Within The Basolateral Amygdala ComplexProgress in Neuro

S.R. Blume, M. Freedberg, J. E. Vantrease, R. Chan, M. Padival, M.J. Record, M. R. DeJoseph, J. H. Urban, J. A. Rosenkranz, 2017, “Sex-And Estrus-Dependent Differences In Rat Basolateral AmygdalaJ. Neurosc.

PL Roubertoux, N. Baril, P. Cau, C. Scajola, A. Ghata, C. Bartoli, P. Bourgeois, J. di Christofaro, S. Tordjman, M. Carlier, 2017, “Differential Brain, Cognitive and Motor Profiles Associated with Partial Trisomy. Modeling Down Syndrome in MiceBehav. Genetics

M. Verma & JS Schneider, 2017, “Strain Specific Effects of Low Level Lead Exposure on Associative Learning and Memory in RatsNeuroToxicology

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