Pain and inflammation
Code: 35250 / 35260

Thermal Place Preference (TPP Test) for Mice & Rats

Two-Temperature Choice Test,

The Ugo Basile Thermal Place Preference Test, or Two-Temperature Choice Test, is an operator independent test which allows monitoring temperature preferences and nociceptive thresholds in rodents (mice and rats), by assessing the rodent's temperature preference.

The TPP Test allows an unrestrained animal (either rat and mouse) to move freely between two 2 compartments set at different temperatures, thus choosing its preferred position (comfort zone).

This behavioural protocol provides data about temperature preferences and nociceptive thresholds associated to both hot and cold stimulation.

Unlike the existing tests, the TPP Test it is operator-independent. Using the traditional hot/cold plates, the researcher measures the reaction time of an animal exposed to a certain temperature or ramp, by marking specific stereotypes while in the "2-Temperatures Choice Test" the nociceptive response is given by the animal choice to move to one or the other environment.

The animal response can be visually observed by the user, and marked manually or on a manual scoring software (ANYmaze Take Note).

In alternative, more detailed information on the animal behaviour can be obtained and recorded automatically via ANYmaze or any other videotracking system.

In the latter case, information will include: time spent in each temperature zone, animal activity, zone trespassing, distance run by the animal (total or by zone)

Additional Info



    Two-environment temperature test  

    Easily monitor thermal place preference and nociceptive thresholds

    Unrestrained animals

    less stress for the animal, less user errors, integrated learned responses to thermal painful and non-painful stimuli

    Based on validated standard Ugo Basile devices

    Can be used as independent Hot/Cold Plate Device and Auxiliary Hot Plate

    Optional Videotracking System for automatic scoring and behavioral tracking

    Bias-free reproducible data

    Versatile device

    Allows flexibility of tests and methods




    Hot/Cold Plate: 4”3 touch-screen (resistive)

    Hot Plate: themoregulator panel


    on the touch-screen

    Power Requirement

    Universal input 85-264 VAC, 50-60Hz, 40W max.

    Sound Level

    < 60 dB (A)

    Operating Temperature

    18° to 25°C




    Hot/Cold Plate: adjustable in the range -5.0 to 65.0°C, with 0.1°C increments

    Hot Plate: from room temperature to 65°C


    +/- 0.1°C


    hot plate, cold plate, ramp (increasing or decreasing)

    Counting Start

    by START button or pedal switch

    Counting Stop

    by pedal switch

    Latency Time Counter

    up to 999.9 seconds, in 0.1 steps

    Data Acquisition

    via X-PAD software, provided

    Data Portability

    by USB flash drive, provided

    TTL Output

    for temperature and counter status


    exported to Text, Excel or Pdf, or saved to cloud to DropBox, OneDrive, GoogleDrive


    exported to Text, Excel or Pdf, or saved to cloud to DropBox, OneDrive, GoogleDrive



    Total Weight


    Shipping Weight

    20Kg approx.


    50(w)x37(d)x47(h)cm – including restrainer

    Packing Dimensions

    68x34x28cm (two boxes


    35250 / 35260 are covered by a 24-month warranty

    Both heat and cold evoke thermosensation, which, if of sufficient intensity, may elicit feelings of pain. Thermosensation is an essential sensory function that is subserved by a variety of transducer molecules.

    Well-established methods are available to measure thermal and mechanical sensitivity in awake behaving rodents, however, they require experimenter manipulations and tend to emphasize reflexive behaviors.

    The TPP (or 2-choice temperature test), is a thermal sensitivity assessment tool designed to emphasize integrated learned responses to thermal painful and non-painful stimuli that are applied dynamically to a surface on which the animal is standing. It documents escape behaviour in awake, unrestrained animals to innocuous and noxious heating of the floor where the animal is located. Animals learn to minimize pain by escaping to the opposite non-heated side; escape latency can be recorded automatically.

    (from 2005 Baliki et al, Molecular Pain 2005, 1:18 doi:10.1186/1744-8069-1-18)




    Thermal Place Preference Test, for Mice and small Rats, standard package, complete. The Package includes a complete Hot/Cold Plate, a complete Auxiliary Hot Plate, and a corridor/TPP tube set for Mice 35250-003


    Thermal Place Preference Test, for Rats, standard package, complete




    Hot/Cold Plate Test, complete


    Auxiliary Hot Plate, complete


    Set of Special Tubes and Catwalk for Mouse, for Thermal Preference set-up 35250, opening 45x95(h)mm


    Set of Special Tubes and Catwalk for Rat, for Thermal Preference set-up 35260, opening 87x110(h)mm




    Software ANYmaze for manual scoring


    ANYmaze videotracking system


    USB camera

    Aziz Moqrich et alia: “Impaired Thermosensation in Mice Lacking TRPV3, a Heat and Camphor Sensor in the Skin” Science 04 Mar 2005: Vol. 307, Issue 5714, pp. 1468-1472


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