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Animal
Mouse, Rat

Hot/Cold Plate - Screening of Thermal Hyperalgesia/Allodynia

Product Code:35300

The hot cold plate test has been the gold standard test for thermal pain tests in rodents for decades. Through the years the devices have been improving and the techniques have become more sophisticated, adding to constant temperature methods also other ones.

The Ugo Basile Hot/Cold plate test includes all the latest technologies necessary to run constant temperature, linear ramping temperature (toward hot or toward cold) and complex temperature ramps thanks to the included X-Pad software.

Moreover, the new model includes a keypad (cabled or optional Bluetooth) to score pain endpoints or stereotyped behaviors.

Temperature range goes from -5° to 65°C and animal holders of different heights are available.

Model
This product warranty can be extended up to 24 Additional Months.
Discover more HERE!

Background

  • Motor (or vocal) responses are normally the endpoints for thermal sensitivity tests and this has been true for hot temperatures and cold temperatures to study hyperalgesia and allodynia.
  • More recently, the introduction of tamping devices, capable to increase the temperature from a starting point to an end point at given time rate, have largely improved the ability to differentiate between hyperalgesic and allodynic responses.
  • The hot/cold plate method is a very traditional one and had the advantage of having unrestrained animals, however, a large variety in scoring methods can be found in the literature, and it depends on the visual scoring of the experimenter herself.
  • Some labs use the first hind paw lick as test endpoints; others use the frequency of licks over a certain time window, other use jumping or vocalizations. 

System components and main features

The Ugo Basile Hot/Cold Plate system comes complete with:

  • An electronic unit with touch screen to set all parameters and save data
  • The Perspex animal restrainer
  • USB port to save data and export them in CSV files
  • A NEW keypad to score endpoints or any other stereotyped behavior (bluetooth optional)
  • A temperature ranges from -5° to 65°C
  • A room ambient temperature sensor
  • A very silent operation, even when the Peltier elements work at full speed to cool down the device quickly
  • A NEW software on the touch screen with several new features, e.g. temperature in °C and °F, an online, an integrated help section, a stop or go option for the end of the ramp mode, a graph showing the plate temperature in real time and a new result screen which is clearer and more comprehensive
  • The new software includes all the animal behaviors that can be scored with the 10 keys available
  • A PC software to build custom temperature ramps and populate your animal vivarium

Additional features include:

  • TTL input/outputs for start/stop and mode command
  • USB port for PC connection
  • COM port for maintenance purposes
  • Ethernet port for support and maintenance (to be used at the factory only)

Feature

Benefit

Fixed, ramping and complex ramp temperature modes

Flexibility for performing many types of pain experiments from allodynia to hyperalgesia

Keypad and pedal for scoring pain animal responses and stereotyped behaviors

Possibility to use the preset 10 keys or modify them (licking, scratching, jumping, grooming, vocalization, rearing, immobility, climbing, shivering, twitching) and save the results in the electronic unit

Broad range of temperatures (-5° to -65°)

Allows to perform any sort of thermal sensitivity experiment, including severe cold allodynia

Ambient temperature sensor is placed outside of the device

Improved system and thermal accuracy

Silent mode of operation or the air dissipation system

Less disturbance to the animal

Control unit and USB key saving

No need to connect a PC

Automatic conversion of data into CSV files for the USB stick

Possibility to open files in Excel

PC software

Possibility to populate your vivarium of animals and to build complex temperature ramps

General

Commands

4,3 inches touch-screen (Resistive)

Read-out

4,3 inches touch-screen (Resistive), PC

Power Requirement

Universal input 100-240 VAC, 50-60Hz, 200W MAX

Sound Level

70 dB

Operating Temperature

18°C to 25°C

Detection

By pedal switch and via numeric key pad

Operation
Plate temperature range -5.0°C to +65°C (in 0.1°C steps)
Accuracy +/- 0.2°C
Mode Fixed temperature, Ramp (increasing or decreasing), Silent, Fast Ramp, Custom Ramp
Start By START button or pedal switch
Stop By STOP button or pedal switch
Experiment duration Max 999.9 seconds
Data Acquisition Via X-PAD software (included)
Data Portability By USB flash drive (included)
Data Input Personalized protocol
Data Output Results table in .csv
TTL Output Temperature, Start, Stop

The advantages of the hot/cold plate are the broad temperature range and hence the possibility to assess both hot and cold allodynia and hyperalgesia. In the last year the tendency has been to use the increasing/decreasing mode more and more frequently to differentiate nociceptor sub-types and hence detect both hyperalgesia and allodynia.

A typical application is during inflammatory pain, as well as in neuropathic pain and to test compounds with anti-nociceptive effects, for example after chronic constriction injury (CCI) or other methods.

The test is mainly used in rodents, but we have seen use in drosophila as well (see literature below, Massingham et al. 2021).

The new model which incorporates now, at no extra cost, the keypad allows for scoring of several behaviors, which before was possible only by videotaping and reviewing the videos, while now it can all be done on-line, during the test.

35300 Hot/Cold Plate includes:

35300-001

Hot/Cold Plate, with USB output, USB flash drive (MY22 model), without animal enclosure

35300-006

Hot/Cold Plate USB wired numeric key-pad (only use with 35300)

 

35100-286

Mouse/Rat Restrainer for Hot/Cold Plate, height 25cm

37215-303

Pedal Switch, complete with cable and connector

E-AU 101

Memory Key, including the user manual and X-pad software

  Power cable according to your Country
Options
35150-002 Additional Hot Plate
35100-285 Animal Restrainer for Hot Plate, 31 cm height
35100-287 Mouse/Rat Restrainer for Hot/Cold Plate, customized height, please specify
35300-005 Hot/Cold Plate wireless USB key-Pad (only use with 35300)
35250-003 Set of Special Tubes and Catwalk for Mouse, for Thermal Preference set-up
35260-002 Set of Special Tubes and Catwalk for Rat, for Thermal Preference set-up
35300-UBC12 UB Care 12 Additional hardware warranty extension 12 months for Hot/Cold Plate 

Eva Mercado et al., 2022, SGK1.1 isoform is involved in nociceptive modulation, offering a protective effect against noxious cold stimulus in a sexually dimorphic manner, Elsevier

Damien C. Boorman and Kevin A. Keay, 2021, M*rphine-Conditioned Placebo Analgesia in Female and Male Rats with Chronic Neuropathic Pain: c-Fos Expression in the Rostral Ventromedial Medulla, Elsevier

S. Al-Mazidi et al., 2017, Blocking of cytokines signalling attenuates evoked and spontaneous neuropathic pain behaviours in the paclitaxel rat model of chemotherapy-induced neuropathy, European Journal of Pain

Petersen et al., 2019, Distinct functions of soluble guanylyl cyclase isoforms NO-GC1 and NO-GC2 in inflammatory and neuropathic pain processing, PAIN

Yasunori Haranishi, Koji Hara, Tadanori TeradaInhibitory, 2021, Inhibitory effect of intrathecally administered AM404, an endocannabin*id reuptake inhibitor, on neuropathic pain in a rat chronic constriction injury modelPharmacological Reports

Koji Hara et al., 2020, Intrathecally administered perampanel alleviates neuropathic and inflammatory pain in rats, Elsevier

Urru Matteo, 2020, Dexpramipex*le blocks Nav1.8 sodium channels and provides analgesia in multiple nociceptive and neuropathic pain models, PAIN

Josephine N. Massingham, 2021, Evaluating Baseline and Sensitised Heat Nociception in Adult Drosophila, Bio Protocol

Moritz Moller et al., 2022, The Role of AlphαSynuclein in Mouse Models of Acute, Inflammatory and Neuropathic Pain, Cells

 

 

 

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