Thermal Place Preference for Rats and Mice

Product Code:35250/35260

Identifies temperature sensitivity and nociceptive pain thresholds. For pathophysiological exploration of animal pain models and pharmacological assessment of analgesic drugs. Often called the Two-Temperature Choice Test.

Easy touch screen controls for setting up and monitoring tests. Fast data portability. Fixed or ramp temperatures for dynamic experiments.

Detailed description +-

Background

Both heat and cold evoke thermosensation may elicit feelings of pain. Thermosensation is an essential sensory function which involves a variety of transducer molecules.
The TPP (or 2-choice temperature test) is a thermal sensitivity assessment tool designed to emphasize integrated learned responses to thermal stimuli.
Escape behavior (latency) in unrestrained animals to innocuous and noxious heating or cooling of the floor can be monitored.
Unlike the traditional cold/hot plate test, this test is operator-independent. Rather than measuring the reaction time of an animal exposed to a certain temperature or ramp, the nociceptive response is provided by the animal as it chooses to move to its preferred position (comfort zone).

Dynamic experiments, easy control

Easy touch-screen controls to set and visually monitor the test.
An unrestrained animal (mouse or rats) can move freely between 2 circular compartments set at different temperatures. Temperature precision is 0.1°C.
Fixed or ramping temperature for dynamic experiments
Choose to make visual observations of animal response or use manual scoring software (ANY-maze Take Note).
Detailed information on animal behaviour can be recorded automatically using ANY-maze software including time spent in each temperature zone, animal activity, zone trespassing, distance run by the animal (total or by zone). Other video tracking software can also be used.
Data portability via the included USB Memory-Key
The Ugo Basile TPP consists of:
- A Hot/Cold Plate
- An additional Hot Plate
- A set including special tubes, and a corridor for either mouse or rat to interconnect the two plates.
Can be used as an independent Hot/Cold plate and Auxiliary Hot Plate.

Features & Benefits +-

Features	Benefits
Two-environment temperature test	Easily monitor thermal place preference and nociceptive thresholds
Unrestrained animals	less stress for the animal, less user errors, integrated learned responses to thermal painful and non-painful stimuli
Based on validated standard Ugo Basile devices	Can be used as independent Hot/Cold Plate Device and Auxiliary Hot Plate
Optional Videotracking System for automatic scoring and behavioral tracking	Bias-free reproducible data
Versatile device	Allows flexibility of tests and methods

Specifications +-

General
Commands	Hot/Cold Plate: 4”3 touch-screen (resistive), Hot Plate: Themoregulator panel
Read-out	On the touch-screen
Power Requirement	Universal input 85-264 VAC, 50-60Hz, 200W max.
Sound Level	Max 60 dB (A)
Operating Temperature	18° to 25°C
Operation
Temperature	Hot/Cold Plate: adjustable in the range -5.0 to 65.0°C, with 0.1°C increments Hot Plate: from room temperature to 65°C
Precision	+/- 0.1°C
Mode	Hot plate, cold plate, ramp (increasing or decreasing)
Counting Start	By START button or pedal switch
Counting Stop	By pedal switch
Latency Time Counter	Up to 999.9 seconds, in 0.1 steps
Data Acquisition	Via X-PAD software, provided
Data Portability	By USB flash drive, provided
TTL Output	For temperature and counter status
Physical
Total Weight	14Kg
Shipping Weight	20Kg approx.
Dimensions	50(w)x37(d)x47(h)cm – including restrainer
Packing Dimensions	68x34x28cm (two boxes)
Warranty
Warranty	35250 / 35260 are covered by a 12-month warranty + 12 after product registration
Ub-Care	Additional UB-Care can be added for other 12 or 24 months

Application +-

Both heat and cold evoke thermosensation, which, if of sufficient intensity, may elicit feelings of pain. Thermosensation is an essential sensory function that is subserved by a variety of transducer molecules.

Well-established methods are available to measure thermal and mechanical sensitivity in awake behaving rodents, however, they require experimenter manipulations and tend to emphasize reflexive behaviors.

The TPP (or 2-choice temperature test), is a thermal sensitivity assessment tool designed to emphasize integrated learned responses to thermal painful and non-painful stimuli that are applied dynamically to a surface on which the animal is standing. It documents escape behaviour in awake, unrestrained animals to innocuous and noxious heating of the floor where the animal is located. Animals learn to minimize pain by escaping to the opposite non-heated side; escape latency can be recorded automatically.

(from 2005 Baliki et al, Molecular Pain 2005, 1:18 doi:10.1186/1744-8069-1-18)

Ordering informations +-

35250 Thermal Place Preference Test, for Mice and small Rats
35250	Thermal Place Preference Test, for Mice and small Rats, standard package, complete. The Package includes a complete Hot/Cold Plate, a complete Auxiliary Hot Plate, and a corridor/TPP tube set for Mice 35250-003
35150	Hot/Cold Plate Test, complete
35150-002	Auxiliary Hot Plate, complete
35250-003	Set of Special Tubes and Catwalk for Mouse, for Thermal Preference set-up 35250, opening 45x95(h)mm
35260 Thermal Place Preference Test, for Rats
35150	Hot/Cold Plate Test, complete
35150-002	Auxiliary Hot Plate, complete
35260-002	Set of Special Tubes and Catwalk for Rat, for Thermal Preference set-up 35260, opening 87x110(h)mm
Optional
60000-TN	Software ANYmaze for manual scoring
60000	ANYmaze videotracking system
47400-040	USB camera

References +-

Aziz Moqrich et alia: “Impaired Thermosensation in Mice Lacking TRPV3, a Heat and Camphor Sensor in the Skin” Science 04 Mar 2005: Vol. 307, Issue 5714, pp. 1468-1472 https://science.sciencemag.org/content/307/5714/1468

Resources & Downloads +-

Instruction Manual Thermal Place Preference Leaflet Hot Cold Plate Leaflet