Orofacial Stimulation Test (Fehrenbacher, Henry, Hargreaves method)

Additional Info

- Mechanical and Thermal nociception assays within the same experiment
- Integrates higher-order brain functions into measurements of orofacial nociception
- Intact vibrissal pad, as the test does not require any vibrissal shaving
- High throughput: up to 16 animals can be tested simultaneously

Water Temperature		can be adjusted from ambient to 70°C
Mechanical Stimulation		3 mechanical stimulators with different wire number, and 1 blank
Oro Software		collects and records beam-break number and duration from up to 16 cages simultaneously
Operating Temperature		10° to 40°C
Sound Level		Negligible
Pollution Degree		≤ 2
PHYSICAL
Net weight		31300                   13Kg 31320                   23Kg 31340                   38Kg
Shipping Weight		31300                   15Kg 31320                   28Kg 31340                   43Kg
WARRANTY	31300 systems are covered by a 24-month warranty

Orofacial pain problems are common and involve structures and mechanisms unique to the trigeminal nerve. Few methods are currently available for orofacial preclinical
research, and none incorporates parallel measurement of mechanical or thermal stimulation within the same experiment.

Moreover, while most of the current assays measure unlearned behaviors, such as flinching or withdrawal reflexes, the new Orofacial Stimulation Test, developed by Fehrenbacher, Henry and Hargreaves, integrates higherorder brain functions into measurements of orofacial nociception.

This innovative approach permits highly integrated nociceptive responses to thermal or mechanical stimulation.

Animals are trained & tested in standard home cages. Tests are performed in the presence of thermal or mechanical stimuli contacting the vibrissal pad. Following treatment to induce hypersensitivity, (e.g., trigeminal ligation or injection) trials are repeated to determine the effect of treatment on feeding behavior/reward. Assay sensitivity (inflammation-induced decreases in feeding behavior and reversal of hypersensitivity by local and systemic administration of analgesics) has been proven (Hargreaves et alia, ms in prep.); the feeding behavior is strongly correlated to mechanical or thermal orofacial nociception, as the animal must contact the stimulator in order to access the food reward.

 

31300	Orofacial Stimulation Test, Complete System for one animal
31320	Orofacial Stimulation Test, Complete System for two animals
31340	Orofacial Stimulation Test, Complete System for four animals
31300-001	Electronic unit (four channels)
31300-002	Additional cage assembly (includes thermal and mechanical stimulators and feeding detector)
31300-003	Circulating water bath
31300-320	Water Distributor (for 2 and 4-cage systems only)
E-AU 041	USB pen-drive, including
	40570-302    Instruction Manual   31300-010 ORO-Software, for data acquisition and analysis from up to 16 cages
OPTIONAL
31300-323	Kit of Mouse adaptors for thermal and mechanical stimulation (for 1 cage)

• J.C. Fehrenbacher et alia: “Characterization of a novel orofacial behavioral assay to assess hyperalgesia to thermal and mechanical stimulation”. (submitted).