Pain and inflammation
Code: 31300

Orofacial Stimulation Test (Fehrenbacher, Henry, Hargreaves method)

The Orofacial Stimulation Test by Ugo Basile is a new method to measures hypersensitivity to thermal or mechanical stimulation of the trigeminal area in rats; animals are trained & tested in standard home cages.

Rats voluntarily contact a thermal or a mechanical stimulator with their vibrissal pad in order to access a food reward. No shaving is required, thus making the test not invasive at all.

This innovative approach permits the parallel measurement of highly integrated nociceptive responses to thermal or mechanical stimulation.

Metrics obtained are the duration of feeding and the number of feeding attempts, measured by interruption of an infrared barrier traversing the opening to the reward. Feeding duration and number of attempts are strongly dependent on changes in the applied thermal or mechanical stimulus.

The ORO-Software included as standard collects and records data from up to 16 cages: Data are shown in real-time both as numeric summary results and in a graphic format. Data are automatically analyzed across time according to an adjustable time window, independently viewable for each of the 16 cages. The results of all the tests are available in a spreadsheet format which can easily be copied to other programs for further analysis.

Adaptors for Mice are available. 

Additional Info

    - Mechanical and Thermal nociception assays within the same experiment


    - Integrates higher-order brain functions into measurements of orofacial nociception


    - Intact vibrissal pad, as the test does not require any vibrissal shaving


    - High throughput: up to 16 animals can be tested simultaneously

    Water Temperature can be adjusted from ambient to 70°C
    Mechanical Stimulation 3 mechanical stimulators with different wire number, and 1 blank
    Oro Software collects and records beam-break number and duration from up to 16 cages simultaneously
    Operating Temperature 10° to 40°C
    Sound Level Negligible
    Pollution Degree ≤ 2
    Net weight

    31300                   13Kg

    31320                   23Kg

    31340                   38Kg

    Shipping Weight

    31300                   15Kg

    31320                   28Kg

    31340                   43Kg

    WARRANTY  31300 systems are covered by a 24-month warranty

    Orofacial pain problems are common and involve structures and mechanisms unique to the trigeminal nerve. Few methods are currently available for orofacial preclinical
    research, and none incorporates parallel measurement of mechanical or thermal stimulation within the same experiment.

    Moreover, while most of the current assays measure unlearned behaviors, such as flinching or withdrawal reflexes, the new Orofacial Stimulation Test, developed by Fehrenbacher, Henry and Hargreaves, integrates higherorder brain functions into measurements of orofacial nociception.

    This innovative approach permits highly integrated nociceptive responses to thermal or mechanical stimulation.

    Animals are trained & tested in standard home cages. Tests are performed in the presence of thermal or mechanical stimuli contacting the vibrissal pad. Following treatment to induce hypersensitivity, (e.g., trigeminal ligation or injection) trials are repeated to determine the effect of treatment on feeding behavior/reward. Assay sensitivity (inflammation-induced decreases in feeding behavior and reversal of hypersensitivity by local and systemic administration of analgesics) has been proven (Hargreaves et alia, ms in prep.); the feeding behavior is strongly correlated to mechanical or thermal orofacial nociception, as the animal must contact the stimulator in order to access the food reward.


    31300 Orofacial Stimulation Test, Complete System for one animal
    31320 Orofacial Stimulation Test, Complete System for two animals
    31340 Orofacial Stimulation Test, Complete System for four animals
    31300-001 Electronic unit (four channels)


    Additional cage assembly (includes thermal and mechanical stimulators and feeding detector)
    31300-003 Circulating water bath
    31300-320 Water Distributor (for 2 and 4-cage systems only)
    E-AU 041 USB drive, including

    40570-302    Instruction Manual  

    31300-010 ORO-Software, for data acquisition and analysis from up to 16 cages

    31300-323 Kit of Mouse adaptors for thermal and mechanical stimulation (for 1 cage)

    • J.C. Fehrenbacher et alia: “Characterization of a novel orofacial behavioral assay to assess hyperalgesia to thermal and mechanical stimulation”. (submitted).

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