Novel Object Recognition

Name: Novel Object Recognition
Brand: Ugo Basile
SKU: 47002/47003

Product Code:47002/47003

Category: Mazes, Tracking

The object recognition test is now among the most commonly used behavioral tests for memory studies in rodents. In its simplest design, an animal is presented with two similar objects during the first session and then one of the two objects is replaced by a new object during a second session or is moved to a different location.

This test is based on the spontaneous tendency of rodents to spend more time exploring a novel object than a familiar one, so that the protocol implies video-tracking or manual measurement of the time spent exploring (or moving toward) the different objects in the different sessions.

Model

Choose a model to get a quote...

Add to Wishlist

Add To Compare

An extended warranty can be added for most Ugo Basile products. You can find this information in the ordering information section of each product.
To learn more about the extended warranty, CLICK HERE

Detailed description +-

Background

The Novel Object Recognition (NOR) test is a widely used behavioral assay in mice to evaluate memory and learning, particularly recognition memory. The test is based on the natural tendency of rodents to explore new objects more than familiar ones. Here's an example of NOR procedure:

Habituation Phase:
- Mice are placed in an empty arena to familiarize themselves with the environment. This phase helps reduce anxiety and baseline exploration behavior.
Training Phase (Familiarization):
- Two identical objects are placed in the arena.
- Mice are allowed to explore these objects for a set amount of time (usually 5-10 minutes).
- The time spent exploring each object is recorded to ensure that there is no inherent preference for one object.
Testing Phase (Novel Object Recognition):
- After a delay (ranging from minutes to hours or even days, depending on the experiment's aim), one of the original objects is replaced with a new, novel object.
- Mice are then reintroduced to the arena and allowed to explore.
- The time spent exploring the novel object versus the familiar one is recorded.

Interpretation of Results:

Higher Novel Object Exploration:
- Mice that remember the familiar object will spend more time exploring the novel one. This indicates intact recognition memory.
Lower Novel Object Exploration:
- Reduced preference for the novel object may suggest impairments in recognition memory, often used to model cognitive deficits or evaluate the efficacy of therapeutic interventions.

Features & Benefits +-

The Novel Object Recognition (NOR) by Ugo Basile are made of resistant plastic material, come in 4 different shapes (sphere, cylinder, cube, pyramid) and in 2 colors (standard white and black) or custom ones.

The Objects come with magnets at the bottom to avoid accidental movement during the test.

Specifications +-

General

Colours: black and white standard; other colours available on request

Dimensions

Dimesions
47002 – Rat NOR	Weight (gr)	Width (mm)	Length (mm)	Diameter (mm)	Height (mm)
Cube	165 gr	80 mm	80 mm	-	80 mm
Sphere	230 gr	-	-	100 mm	87,5 mm
Pyramid	130 gr	110 mm base 33 mm top	110 mm base 33 mm top	-	80 mm
Cylinder	130 gr	-	-	80 mm	80 mm

47003 – Mouse NOR	Weight (gr)	Width (mm).	Length (mm)	Diameter (mm)	Height (mm)
Cube	35 gr	40 mm	40 mm	-	40 mm
Sphere	35 gr	-	-	50 mm	43,7 mm
Pyramid	35 gr	55 mm base 18 mm top	55 mm base 18 mm top	-	40 mm
Cylinder	30 gr	-	-	40 mm	40 mm

Application +-

This test is based on the spontaneous tendency of rodents to spend more time exploring a novel object than a familiar one.

The Novel Object Recognition (NOR) test is a widely used behavioral assay in neuroscience to assess recognition memory, a form of declarative memory. It leverages rodents' natural preference for novelty to evaluate cognitive functions, particularly in relation to memory, learning, and brain function. Here are the key applications of the NOR test in behavioral neuroscience:

Memory and Cognitive Function:

Recognition Memory: The NOR test is specifically designed to evaluate non-spatial recognition memory, which involves distinguishing between familiar and novel objects.
Assessment of Memory Impairment: It is commonly used to study memory deficits in models of neurodegenerative diseases, aging, and brain injury.
Short-term and Long-term Memory: By varying the delay between the training and test phases, the test can assess short-term versus long-term memory processes.

Neurodegenerative Disease Models:

Alzheimer’s Disease (AD): The test is extensively used to study memory impairment in rodent models of AD and to evaluate potential therapeutic interventions targeting memory deficits.
Parkinson’s Disease and Huntington’s Disease: The NOR test is also relevant in studying memory impairments associated with other neurodegenerative conditions.

Pharmacological and Genetic Studies:

Drug Development: It serves as a tool to evaluate the efficacy of drugs targeting cognitive impairments, such as acetylcholinesterase inhibitors, NMDA receptor modulators, and other nootropic agents.
Genetic Manipulations: The test is used to study the role of specific genes in memory and cognitive function through knockout, knock-in, or transgenic rodent models.

Neural Circuitry and Molecular Mechanisms:

Hippocampus and Prefrontal Cortex Function: The NOR test is often combined with lesions, optogenetics, or pharmacological interventions to dissect the role of specific brain regions and circuits.

Behavioral Phenotyping

Rodent Strain Comparisons: The NOR test is used to characterize cognitive profiles across different rodent strains to identify innate differences or genetic susceptibilities.

The NOR test’s simplicity, cost-effectiveness, and ability to provide robust data make it a cornerstone in behavioral neuroscience, particularly in studies aimed at understanding memory and cognitive processes in health and disease.

Ordering informations +-

Product
47002	Set of 8 objects for NOR for rats: squares, pyramids, cylinders and spheres (8cm; 4 black + 4 white)
47003	Set of 8 objects for NOR: for mice: squares, pyramids, cylinders and spheres (4cm; 4 black + 4 white)
47002-W	Set of 4 objects for NOR for rats: piramid, cube, cylinder, sphere (8cm; 4 white)
47002-BK	Set of 4 objects for NOR for rats: piramid, cube, cylinder, sphere (8cm; 4 black)
47003-W	Set of 4 objects for NOR for mice: piramid, cube, cylinder, sphere (4cm; 4 white)
47003-BK	Set of 4 objects for NOR for mice: piramid, cube, cylinder, sphere (4cm; 4 black)
Videotracking
60000	ANY-maze Software
47400-040	B/W USB Camera, including day&night 2.8-12.0mm varifocal lens, 5m USB cable and ceiling support