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Mouse, Rat

Fear Conditioning System

Product Code:46000

Complete system for Cued and Contextual fear conditioning tests using rodents. For neuro-behavioral, pharmacological and genetic studies. Automatic detection of freezing response using ANY-maze special Fear Conditioning. Fast set up and assisted setting up of experiment protocols. Software controlled light, sound and shock. Easy system expansion

Easy control, easy set up, easy expansion. Automatically detects the freezing response (Conditional Response), even in total darkness. Reporting information includes total freezing time, number and duration of freezing episodes, latency times between stimuli and freezing.

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This product warranty can be extended up to 24 Additional Months.
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  • Our system automates the two most common fear conditioning paradigms in mice or rats using Pavlovian associative learning experiments: Cued and Contextual Fear Conditioning. It works as follows:
    • Cued Fear Conditioning: Single-frequency sounds are delivered as a neutral or conditioned stimulus (CS). The electric current from the grid floor is delivered as the aversive, or unconditioned stimulus (US). 
    • Contextual Fear Conditioning: The visual appearance of the cage serves as the conditioned stimulus (CS). The context kit includes a set of colored floors and patterned walls. The animal associates the electric shock to the environment (context) in which it was received.
    • Fear Conditioning (FC) is learned rapidly (usually only one trial) producing a stable and long-lasting behavioral change. This is useful for drug discovery, neurobehavioral, pharmacological and genetic studies.

 Easy control, easy set up, easy expansion

  • Automatically detects the freezing response (Conditional Response), even in total darkness. Reporting information includes total freezing time, number and duration of freezing episodes, latency times between stimuli and freezing. 
  • ANY-maze (the brain of our FC-system) controls the experimental settings, including light, sound (in the range 100Hz-18KHz; 55-100dB or white noise), and shock (constant current pre-settable from 0.1 to 3.0mA in 0.1mA steps). To facilitate easy set up, ANY-maze will set up protocol items for you. Simply select the mode in the Protocol list named ‘Ugo Basile Fear Conditioning mode’ and answer the questions about your cage set up.
  • A typical Fear Conditioning (FC) System consists of:
    • FC-Unit – includes a Sound-Attenuating chamber, with noiseless ventilating fan, a dual (visible and I.R.) light, a loudspeaker and an USB-camera. Each FC-Unit has an individual controller on-board. Wide-angle lenses and IR filters are included.
    • FC-Cage – for mouse or rat, with electrified floor and context kit (3 striped, 3 checkered, 3 grey walls and 3 plastic floors (white, black, grey). See ordering information for the range of available cages.
    • ANY-maze video tracking software (special FC version available) 
    • Extremely simple to set-up: just plug a USB cable into a single USB port on the FC-Unit.
  • Optional models for tethered animals are available.
  • Expand your system easily. Each FC-Unit is individually connected to ANY-maze, with virtually no limit in number.
  • Upgrade path available if you own a previous version of our FC-System. Ask for details!



New Features

AUTOMATIC detection of FREEZING also in total darkness

Extremely simple to set-up: just plug a USB cable into a single USB port on the FC-Unit

Specific versions for rats or mice

Multiple Cage Set-up, with virtually no limit in number

Select the most suitable cage: two sizes, available with optional features, such as specific versions for tethered animals, etc.

ANY-maze controls all phases of the test, managing the experimental parameters, detecting the animal respon­se and analyzing the experimental data.

Camera IR (lens 2.1mm and 4.3mm)
Active Silent Ventilation Yes
Read-Out USB to PC, TTL I/O



Constant current

Shock Intensity

from 0.1 to 2.9mA, in 0.1mA steps

Shock Duration

Duration: from 0.5 to 99 seconds


Dimmable visible and IR


500Hz-18KHz, 100dB ± 3dB

White Noise 77dB ± 3dB


100-240 VAC, 50/60 Hz

Operating Temperature

10°C to 40°C; 5% to 95% RH (non-condensing)

Sound Level

Negligible (sound cue excluded)

Pollution Degree

≤ 2


Dimension Outer Size Internal Size



Mouse Cage 24(w)x20(d)x30(h)cm 17x17x25(h)cm
Rat Cage 33(w)x29(d)x41(h)cm 25.5x25.5x36(h)cm
Mouse Cage XL 33(w)x29(d)x41(h)cm 25.5x25.5x36(h)cm



Shipping Weight



Warranty Fear Conditioning is covered by a 12-month warranty + 12 after product registration
UB-Care Additional UB-Care can be added for other 12 or 24 months


PC Requirements
PC Requirements

Minimum specification

Recommended specification

Best system

Windows version

XP service pack 3

Windows 10

Windows 10


Pentium Dual-core

Core i3

Core i7





Hard drive

1GB available




1366 x 768px

1600 x 900px

1920 x 1080px


Not applicable

Not applicable

PCi express ports

If you're planning to track in multiple apparatus, or track for long periods (> 12 hours), or include large numbers (> 500) of animals in experiments then we recommend you read the whitepaper Choosing a computer for ANY-maze which discusses various technical considerations that you may need to take into

Fear Conditioning is a quick and reliable method to assess memory in rodents.
When rodents are exposed to fearful stimuli, they respond, among the others, with immobility behavior, also called “freezing”. All different Fear Conditioning procedures imply the measurement of the freezing response (i.e. the fraction of time spent immobile).
A rodent can learn to fear a previous neutral stimulus if that has been associated with an instinctively aversive one (such as an electric shock), or, in other terms, after having been conditioned.
After the conditioning has occurred, and the two stimuli have become associated, the animal is presented the neutral stimulus and, as a consequence, it will freeze. Freezing is commonly defined as complete immobility, apart from respiratory movements.
The time spent freezing is indicative of undergone memory and learning processes, which are at the basis of the association between neutral and aversive stimuli.
Fear Conditioning is a sensitive and quick test, which requires very little training (usually only one trial) to the animals. It is therefore a valuable tool for basic behavior research, as well as for high throughput and drug discovery studies. Moreover, the conditioning established during the Fear Conditioning procedure can be very strong (i.e. long-lasting), allowing for long term experiments.

Fear Conditioning System


Fear-Conditioning Unit, including Isolation Cubicle 46000-596 (inside dimensions 48.5(w)x38.5(d)x48.5(h)cm), dual visible/I.R. light 46000-325, speaker 46000-165, controller on-board 46000-105, camera 47400-035, cables and power supply. System to be completed with software ANY-maze (full license or FC-version) and mouse or rat cage


Bundle of 2 FC-Units


Bundle of 3 FC-Units


Bundle of 4 FC-Units

Fear Conditioning - Cages


Rat Cage, I.D. 26x26x30(h)cm


Mouse Cage, I.D. 17x17x25(h)cm


Mouse XL Cage, I.D. 26x26x30(h)cm, with mouse floor

46004-D01 Mouse Cage with Front Door
46005 Fear Conditioning Cage for Observational Learning



ANY-maze software, FC-specific version


ANY-maze software, full license


ANY-maze or higher is necessary to manage Ugo Basile FC 2.1: upgrades are available



Sound Attenuating Cubicle (48.5(w)x38.5(d)x48.5(h)cm)


Controller on-board




Dual (visible/I.R.) Light


USB Camera

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