NEW Fear Conditioning System - ANYmaze
Code: 46001

NEW Fear Conditioning System - ANYmaze Featured

The UGO BASILE ANY-maze controlled Fear Conditioning System  (Fear Conditioning 2.1) automates the two most common fear conditioning paradigms: Contextual Fear Conditioning and Cued fear Conditioning

The detection of freezing is automated and based on video analysis.

The Ugo Basile Fear Conditioning systems are designed to run Pavlovian conditioning experiments on mice or rats. Single-frequency sounds are delivered as neutral or conditioned stimulus (CS). Experimental procedures which contemplate the sound as CS are usually referred to as Cued Fear Conditioning. Electric current from the grid floor is delivered as the naturally aversive, or unconditioned stimulus (US).

Tests are run in an animal cage with a grid floor, whose appearance can be easily altered by attaching patterned contexts on the walls and floor. This allows for the investigation of Contextual Fear Conditioning, in which the CS is not the sound but the visual appearance of the cage (i.e. the context) itself. In this case the animal associates the electric shock to the environment where it was received.

A typical FC-System consists of:

  • an FC-Unit, encompassing a Sound-Attenuating Box, with ventilating fan, a dual (visible/I.R.) light, a speaker & a USB-camera. Each FC-Unit has an individual controller on-board
  • an FC-Cage, for mouse or rat, (see ordering information for available models), with electrified floor & context kit (3 floors and 3 sets of patterned walls)
  • ANY-maze software (FC or full license, from version 6.0, upgrade available)

Animal Cages with Electrified Grid Floor are available in the following models:

  • 46002 Rat Cage, I.D.: 26x26x30(h)cm
  • 46003 Mouse Cage, I.D.: 17x17x25(h)cm
  • 46004 Mouse XL Cage, I.D. 26x26x30(h)cm, with mouse floor

Optional models for tethered animals are available.

Multiple systems, with virtually no limit, are easily assembled by multiplying the number of FC-Units and FC-cages, with no additional multiplexer or interface required.

anymaze logo

ANY-maze controls the shocker, the sound generator and the light (I.R. and visible), and automatically detects the animal freezing, reporting information such as: total freezing time, number and duration of freezing episodes, latency times between stimuli and freezing. 

NEW to the 2.1 version:

  • Extremely simple to set-up: just plug a USB cable into a single USB port on the FC-Unit
  • ANY-maze controls all phases of the test, managing the experimental parameters, detecting the animal response and analyzing the experimental data
  • An Ugo Basile Fear Conditioning mode is provided in ANY-maze protocol list to facilitate the set-up: by asking some questions about the way you want to set up the cages, the software creates lots of the protocol items for you!
punto esclamativo blu 1

You own a previous version of our FC-System? No worries: you can easily upgrade it to the new version, and/or add extra units. Ask for details!

Additional Info

    Main Features NEW to 2.1 version
    AUTOMATIC detection of FREEZING also in total darkness Extremely simple to set-up: just plug a USB cable into a single USB port on the FC-Unit
    Specific versions for rats or mice Multiple Cage Set-up, with virtually no limit in number
    Select the most suitable cage: two sizes, available with optional features, such as specific versions for tethered animals, etc. ANY-maze controls all phases of the test, managing the experimental parameters, detecting the animal respon­se and analyzing the experimental data.
    Shock Constant current
    Shock Intensity from 0.1 to 2.9 mA, in 0.1 mA steps
    Shock Duration Duration: from 0.5 to 99 seconds
    Visible Light 0-100%
    I.R. Light 0-100%

    100Hz-18KHz, 0-100% (100%0100dB ±3dB)

    and white noise 0-100% (100%=77dB ±3dB)

    Power  80V/240Vac, 50/60 Hz
    Operating Temperature 10° to 40°C
    Sound Level Negligible (sound cue excluded)
    Pollution Degree ≤ 2
    Number of Cages no limits

    Cubicle 55(d)x60(w)x57(h)cm

    Mouse Cage 24(w)x20(d)x30(h)cm, ID 17x17x25(h)cm

    Rat Cage 33(w)x29(d)x41(h)cm, ID 25.5x25.5x36(h)cm

    Mouse Cage XL 33(w)x29(d)x41(h)cm, ID 25.5x25.5x36(h)cm

    Weight 35Kg, for each cage set
    Shipping Weight 40Kg, for each cage set
    Packing Dimensions Wooden crate 82x71x57cm for each cage
    Warranty 48000 is covered by a 24-month warranty

    PC Requirements

    ANY-maze will run on almost any Windows based computer: ANY-maze runs under all versions of Windows from XP to Windows 10.

    If you're only planning to track a single animal then there aren't really any special requirements and almost any computer built in the last five years should work fine. However, if you want to track in multiple set-up simultaneously then you may need a more powerful machine.

      Minimum specification Recommended specification Power system
    Windows version XP service pack 3 Windows 10 Windows 10
    Processor Pentium Dual-core Core i3 Core i7
    RAM 1GB 4GB 8GB
    Hard drive 1GB available 500GB 1TB
    Display 1366 x 768 24-bit colour 1600 x 900 32-bit colour 1920 x 1080 32-bit colour
    USB ports 1 available 2 available 4 available
    Expansion Not applicable Not applicable PCi express ports

    If you're planning to track in multiple apparatus, or track for long periods (> 12 hours), or include large numbers (> 500) of animals in experiments then we recommend you read the whitepaper Choosing a computer for ANY-maze which discusses various technical considerations that you may need to take into 

    Fear Conditioning is a quick and reliable method to assess memory in rodents.

    When rodents are exposed to fearful stimuli, they respond, among the others, with immobility behavior, also called “freezing”. All different Fear Conditioning procedures imply the measurement of the freezing response (i.e. the fraction of time spent immobile).

    A rodent can learn to fear a previous neutral stimulus if that has been associated with an instinctively aversive one (such as an electric shock), or, in other terms, after having been conditioned.

    After the conditioning has occurred, and the two stimuli have become associated, the animal is presented the neutral stimulus and, as a consequence, it will freeze. Freezing is commonly defined as complete immobility, apart from respiratory movements.

    The time spent freezing is indicative of undergone memory and learning processes, which are at the basis of the association between neutral and aversive stimuli.

    Fear Conditioning is a sensitive and quick test, which requires very little training (usually only one trial) to the animals. It is therefore a valuable tool for basic behavior research, as well as for high throughput and drug discovery studies. Moreover, the conditioning established during the Fear Conditioning procedure can be very strong (i.e. long-lasting), allowing for long term experiments.


    46001 Fear-Conditioning Unit, including Isolation Cubicle 46000-596 (inside dimensions 48.5(w)x38.5(d)x48.5(h)cm), dual visible/I.R. light 46000-325, speaker 46000-165, controller on-board 46000-105, camera 47400-035, cables and power supply. System to be completed with software ANYmaze (full license or FC-versione) and mouse or rat cage
    46001-2 Bundle of 2 FC-Units
    46001-3 Bundle of 2 FC-Units
    46001-4 Bundle of 4 FC-Units
    46002 Rat Cage, I.D. 25.5x25.5x36(h) cm
    46003 Mouse Cage, I.D. 17x17x25(h) cm
    46004 Mouse XL Cage, I.D. 25.5x25.5x36(h) cm, with mouse floor
    60000-FC ANY-maze software, FC-specific version
    60000 ANY-maze software, full license
    NOTE: ANY-MAZE 6.0 or higher is necessary to manager Ugo Basile FC 2.1: upgrades are available
    46000-596 Sound Attenuating Cubicle (48.5(w)x38.5(d)x48.5(h)cm)
    46000-105 Controller on-board
    46000-165 Speaker
    46000-325 Dual (visible/I.R.) Light
    47400-035 USB Camera

Related items by application