IR-transparent mazes for video-tracking
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Animal
Mouse, Rat
Thermal Place Preference for Rats
Product Code:35360
Category: Pain and Inflammation
The Thermal Place Preference test can be considered an evolution of the Hot/Cold plate test and it allows monitoring of temperature preferences by measuring the time spent in two compartments set at two different temperatures.
This two temperature nociception test can be used in a mouse or rat and is composed of a standard hot/cold plate and of an hot plate, connected by a corridor. It is operator independent, which represents a big advantage over the hot/cold plate and also the endpoint to be measured is much more objective.
Moreover, it can be automated by using videotracking which scores the time, distance, etc, spent in each of the two compartments and corridor. Temperature range goes from -5° to 65° and animal holders of different heights are available.
Background
- The hot/cold plate test has some limitations, like the experimenter bias, the lack of consensus around the pain threshold endpoints (first lick latency, frequency of lick, jumping, vocalization), which the Temperature Place Preference overcomes.
- In addition to allowing the assessment of temperature preference behaviors, it also automatizes the scoring of the nociceptive behavior if video-tracking is used and makes the scoring more objective anyway, as no action by the operator is required. .
System components and main features
- The Ugo Basile Thermal Place Preference includes:
- A Hot/Cold Plate system
- A corridor (choose version for rats or mice)
- A hot plate
- An electronic unit with touch screen to set all parameters and save data
- The Perspex animal restrainers
- USB port to save data and export them in CSV files
- A NEW keypad to score endpoints or any other stereotyped behavior (bluetooth optional)
- A temperature range from -5° to 65° C
- A room ambient temperature sensor
- A very silent operation, even when the peltier elements work at full speed to cool down the device quickly
- A NEW software on the touch screen with several new features, e.g. temperature in C° and °F, an online, an integrated help section, a stop or go option for the end of the ramp mode, a graph showing the plate temperature in real time and a new result screen which is clearer and more comprehensive
- The new software includes all the animal behaviors that can be scored with the 9 keys available
- A PC software to build custom temperature ramps and populate your animal vivarium
- Additional features include:
- TTL input/outputs for start/stop and mode command
- USB port for PC connection
- COM port for maintenance purposes
- Ethernet port for support and maintenance (to be used at the factory only)
- Optionally, a system with tripod, camera and video-tracking can be purchased to automatize the time spent in each plate, the distance travelled, the number of entries/exits, etc.
Please note: slight variations in the coloration of the plates may occur. This is due to the manufacturing process and does not affect the procedure or the outcome of the experiment in any way.
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Features |
Benefits |
|---|---|
|
Standard Hot/Cold plate connected to a hot plate |
Fixed, ramping and complex ramp temperature modes, for flexibility in performing many types of pain experiments from temperature preference to allodynia and hyperalgesia |
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Keypad and pedal for scoring pain animal responses and stereotyped behaviours and save the results in the electronic unit |
Possibility to use and save the preset 9 keys or modify them (licking, scratching, jumping, grooming, vocalization, rearing, immobility, climbing, shivering, twitching |
|
Broad ramp of temperatures (-5°-65°C) |
Allows for any sort of thermal sensitivity experiment, including severe cold allodynia |
|
Ambient temperature sensor is placed outside of the device |
Accuracy is much higher |
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Silent mode of operation or the air dissipation system |
Less disturbance to the animal |
| Control unit and USB key saving | No need to connect a PC |
| Automatic conversion of data into CSV files for the USB stick | Possibility to open files in Excel |
| PC software | Possibility to populate your vivarium of animals and to build complex temperature ramps |
| Optional complete video-tracking software with tripod and camera | Allows for extended and automated data parameters, video saving and no need for the experimenter to be present during the test and to run multiple animals at the same time (with multiple cameras) |
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General | |
|---|---|
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Commands |
Hot/Cold Plate: 4”3 touch-screen (resistive), Hot Plate: Themoregulator panel |
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Read-out |
On the touch-screen |
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Power Requirement |
Universal input 85-264 VAC, 50-60Hz, 200W max. |
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Sound Level |
Max 60 dB (A) |
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Operating Temperature |
18° to 25°C |
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Operation | |
|
Temperature |
Hot/Cold Plate: adjustable in the range -5.0 to 65.0°C, with 0.1°C increments Hot Plate: from room temperature to 65°C |
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Precision |
+/- 0.1°C |
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Mode |
Hot plate, cold plate, ramp (increasing or decreasing) |
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Counting Start |
By START button or pedal switch |
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Counting Stop |
By pedal switch |
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Latency Time Counter |
Up to 999.9 seconds, in 0.1 steps |
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Data Acquisition |
Via X-PAD software, provided |
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Data Portability |
By USB flash drive, provided |
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TTL Output |
For temperature and counter status |
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Physical | |
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Total Weight |
14Kg |
|
Shipping Weight |
20Kg approx. |
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Dimensions |
50(w)x37(d)x47(h)cm – including restrainer |
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Packing Dimensions |
68x34x28cm (two boxes) |
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Warranty | |
| Warranty | 35350 / 35360 are covered by a 12-month warranty + 12 after product registration |
| Ub-Care | Additional UB-Care can be added for other 12 or 24 months |
The thermal place preference test has been shown to differentiate behavioral responses to cold stimuli between sham and chronic constriction injury (CCI) rodents with multiple animals tested simultaneously. Also, the effect of antinociceptive drugs has been differentiated by this test in a high throughput manner in several models of neuropathic pain.
A typical application is during inflammatory pain, as well as in neuropathic pain and to test compounds with antinociceptive effects, for example after chronic constriction injury (CCI) or other methods.
In general, the Temperature Place Preference test has been designed and is used for all application that require to finely assess thermal sensitivity in rodents.
Typically, one plate is set at a fixed temperature 5°C or 50°C and the other at a temperature close to the ambient one, e.g., 25°C. Hyper- or hyposensitivity is assessed in this way to study pathophysiological aspects of animal pain models and for the pharmacological assessment of analgesic drugs.
The NEW model which incorporates now, at no extra cost, the keypad allows for scoring of several behaviors, which before was possible only by videotaping and reviewing the videos, while now it can all be done on-line, during the test.
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35350 Thermal Place Preference Test, for Mice and small Rats - What's in the box | |
|---|---|
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35300 |
Hot/Cold Plate Test, complete with numeric keypad for behavioural scoring |
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35150-002 |
Auxiliary Hot Plate |
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35250-003 |
Set of Special Tubes and Catwalk for Mouse, for Thermal Preference set-up 35250, opening 45x95(h)mm |
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Please note: slight variations in the coloration of the plates may occur. This is due to the manufacturing process and does not affect the procedure or the outcome of the experiment in any way. |
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|
35360 Thermal Place Preference Test, for Rats - What's in the box | |
|---|---|
|
35300 |
Hot/Cold Plate Test, complete with numeric keypad for behavioural scoring |
|
35150-002 |
Auxiliary Hot Plate |
|
35260-002 |
Set of Special Tubes and Catwalk for Rat, for Thermal Preference set-up 35260, opening 87x110(h)mm |
|
Please note: slight variations in the coloration of the plates may occur. This is due to the manufacturing process and does not affect the procedure or the outcome of the experiment in any way. |
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Optional | |
|---|---|
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60000-TN |
AnyMaze, TakeNote software for manual scoring |
|
60000 |
ANY-maze, flexible video tracking system designed to automate testing in behavioural experiments. Full License, including lifelong support and updates |
|
47400-040 |
USB camera |
| E-AU 119 | Table tripod for cameras |
David Balayssac et. al., 2014, "Assessment of thermal sensitivity in rats using the thermal place preference test", Behavioural Pharmacology
Greg A Weir et. al., 2017, "Using an engineered glutamate-gated chloride channel to silence sensory neurons and treat neuropathic pain at the source", BRAIN
Aida Castellanos et. al., 2020, "TRESK background K+ channel deletion selectively uncovers enhanced mechanical and cold sensitivity", The Journal of Physiology
Liam J. Peck et. al., 2021, "Studying Independent Kcna6 Knock-out Mice Reveals Toxicity of Exogenous LacZ to Central Nociceptor Terminals and Differential Effects of Kv1.6 on Acute and Neuropathic Pain Sensation", J. Neurosci.
Sydney E Lee et. al., 2022, "Anxiety-like behaviors in mice unmasked: Revealing sex differences in anxiety using a novel light-heat conflict test", Research Gate
D. Balayssac et. al., 2014, "Assessment of thermal sensitivity in rats using the thermal place preference test: description and application in the study of oxaliplatin-induced acute thermal hypersensitivity and inflammatory pain models", Behavioural Pharmacology
