LMD Lesion Making Device, for localized electrolytic lesions

Additional Info

FEATURES	BENEFIT
Current generator protected against short circuit	Prevents damage to electronics, guarantees safety
Wide current output range (10uA-99mA) and duration (1-99 s)	Allows for virtually any procedure
Three Operating Modes	Continuous, Stand-by and Timed
Violation LED indicator	Indicates if compliance is violated

Operation
Lesion Making Device	DC Constant Current
Current Range	from 10 µA to 99 mA
Compliance Voltage	200 V DC
Max. Electrode Resistance	20 MOhm (10µA) down to 2 KOhm (100mA)
Power Monitoring	via red LED
Current Monitoring	via green LED
Violation Monitoring	via yellow LED
Power Requirement	115 or 230 V, 50/60 Hz, 20 W max.
Operating Temperature	10° to 40° C
Sound Level	<45dB
Physical
Total Weight	1.4Kg
Shipping Weight	3.7Kg approx.
Dimensions	27(w)x25(d)x13(h)cm
Packing Dimensions	45x34x26cm
Warranty
Warranty	53500 is covered by a 24-month warranty.

 

 

 

 

D

The surgically or electrically induced lesion has served as an important tool in the exper-imental search for function in the CNS. Its value has derived in part from the simplicity with which it can be used to study neural mechanisms of behaviour at a basic level.

The advent of the stereotaxic technique, moreover, allowed researchers to produce dis-cretely placed lesions with consistency, especially in sub-cortical structures of the brain.

The strength of the lesion technique resides also in the variety of ways with which to ap-ply it. Manipulating the type of lesion (DC, RF, knife cut, etc.), its size, the type of elec-trode, the angle of entry, and so on, should continue to expose critically important as-pects of neural functions because of the different effects that are produced.

It is no coincidence that the history of the development of these techniques is closely tied to the recent history of theories regarding localization of function in the brain. The tradi-tional view, whose basic tenets are that (1) the functions are represented in discrete brain structures and that (2) the lesions disrupt function by removal of functional tissue in circumscribed sites, have been recently challenged by growing evidence of the importance of secondary changes.

These are induced by a lesion, both directly (necrosis, anterograde and retrograde de-generation) and indirectly (transneuronal degeneration, regeneration and sprouting, al-teration of neurochemical pools, vascular disruption) and may comprise the more signifi-cal neurological changes which can account for alteration of behaviour in a lesion exper-iment.

New strategy of research utilizing lesions is proposed, suggesting that greater emphasis be placed on the a posteriori assessment of secondary changes in the brain as they are correlated with changes in behaviour.

 

53500	Lesion Making Device, complete with standard accessories
53500-310	Set of 3 output plugs
E-AU 041	USB pen drive, including:  53500-302    Instruction Manual
Optional
53500-325	Set of 2 Microelectrodes SS Custom FHC for Lesion Making Device, complete with black and red banana plug, and 1 meter cable
UE(KK1)	Microelectrode SS Custom FHC (no wires)

Bibliography on 53500

• A rat model for pituitary stalk electric lesion-induced central diabetes insipidus: application of 3D printing and further outcome assessments Z FENG, Y OU, M ZHOU, G WU, Y BAO, B QIU, S QI - Experimental Animals 2018 (Rats)
• Binaural responses in the auditory midbrain of chicken (Gallus gallus) R Aralla, G Ashida, C Köppl - 2018 (Chicken)
• Functional ectopic neural lobe increases GAP-43 expression via PI3 K/AKT pathways to alleviate central diabetes insipidus after pituitary stalk lesion in rats Z Feng, Y Ou, M Zhou, G Wu, L Ma, Y Zhang, Y Liu… - Neuroscience Letters, 2018
• Effect of endogenous arginine-vasopressin arising from the paraventricular nucleus on learning and memory functions in vascular dementia model rats CY Li, L Zhang, J Li, CL Qi, DY Li, X Liu, X Qu, 2017
• Stereotaxic Surgery for Suprachiasmatic Nucleus Lesions in Mice K Shimizu, Y Fukada, 2017
• Cold-induced thermogenesis and inflammation-associated cold-seeking behavior are represented by different dorsomedial hypothalamic sites: a three-dimensional … SP Wanner, MC Almeida, YP Shimansky, DL Oliveira… - Journal of Neuroscience, 2017
• An intra-amygdala circuit specifically regulates social fear learning RC Twining, JE Vantrease, S Love, M Padival… - Nature Neuroscience, 2017
• Left–right functional asymmetry of ventral hippocampus depends on aversiveness of situations Y Sakaguchi, Y Sakurai - Behavioural Brain Research, 2017
•  The area postrema (AP) and the parabrachial nucleus (PBN) are important sites for salmon calcitonin (sCT) to decrease evoked phasic dopamine release in the … L Whiting, JE McCutcheon, CN Neuner-Boyle… - Physiology & Behavior, 2017
• V. Campese et alia: “Modulation of Instrumental Responding by a Conditioned Threat Stimulus Requires Lateral and Central Amygdala” Frontiers in Behav. Neurosc. 9(293), 2015
• S.M. Fortin et alia: “Sampling Phasic Dopamine Signaling with Fast-Scan Cyclic Voltammetry in Awake, Behaving Rats” Current Protocols in Neuroscience, UNIT 7.25, published online 5 Jan 2015
• S.M. Fortin et alia: “Sampling Phasic Dopamine Signaling with Fast-Scan Cyclic Voltammetry in Awake, Behaving Rats” Current Protocols in Neuroscience, UNIT 7.25, published online 5 Jan 2015
• V.D. Campese et alia: “Lesions of lateral or central amygdala abolish aversive Pavlovian-to-instrumental transfer in rats” front Behav Neurosci. 8: 161, 2014
• M.G. McCue et alia: “Medial Amygdala Lesions Selectively Block Aversive Pavlovian–Instrumental Transfer in Rats” front Behav Neurosci. 8: 329, 2014
• Stroobants et alia: “PIncreased gait variability in mice with small cerebellar cortex lesions and normal rotarod performance” Behav. Brain Res. 241: 32-37, 2013
• L.B. Cruz et alia: “Effect of the bone marrow cell transplantation on elevated plus-maze perfor­mance in hippocampal-injured mice” Behav. Brain Res. available online Apr. 2013
• M.E. Wang: “Long-Term Stabilization of Place Cell Remapping Produced by a Fearful Experience” J. Neurosci. 32(45): 15802-15814, 2012
• M.B. Gomes et alia: “Glucose levels Observed in Daily Clinical Practice induce Endothelial Dy­sfunction in the Rabbit Macro- and Microcircula­tion” Fund. & Clin. Pharmacol. 18 (3), 2004
• C. Hamani et alia: “Bilateral Anterior Thalamic Nucleus Lesions and High-frequency Stimula­tion Are Protective against Pilocarpine-induced Seizures and Status Epilepticus” Neurosurgery, 54 (1): 191-197, 2004
• T. Lee and J.J. Kim: “Differential Effects of Cere­bellar, Amygdalar, and Hippocampal Lesions on Classical Eyeblink Conditioning in Rats” J. Neuro­science 24 (13): 3242-3250, 2004
• K.C. Bicego and L.G.S. Branco: “Discrete Electro­lytic lesion of the Preoptic Area Prevents LPS-Induced Behavioral Fever in Toads” J. Exper. Biol. 205: 3513-3518, 2002