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What are the main confounding factors in rodent behavioral experiments?

In virtually all the “well-established tests for assessing various behavioral readouts subtle aspects can influence measurements. Features such as housing conditions, conditions of testing, and the sex and strain of the animals can all have effects on tests of behavior. In the conduct of behavior testing, it is important to keep these features in mind to ensure the reliability and reproducibility of results” (Saré et al. 2021 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073298/ )