The three-chamber test is the classic test to assess social behavior and social memory in rodents. This may range from autism disease to dominance tests and social novelty. Additional applications include sociability differences between genders, strains and any other variable, which may end up with a social preference.

The three-chamber sociability test has been used to assess social behaviour in mice with altered GTF2I levels, in order to evaluate the impact of GTF2I dosage on social interactions and preferences.