Tissue baths, Transducers, Recorders

14900 - Superfusion System

Superfusion System Superfusion System

The Ugo Basile 14900 Superfusion System is a semi-automated device to study the release of neurotransmitters in synaptosome samples. It is a development of the instrument originally developed in Prof. Raiteri's laboratory, where about 300 papers have been published exploiting the technique.

The 14900 Superfusion System consists of 12 parallel open superfusion chambers with 12 upper reservoirs, all thermo-stated by a water-jacket. Pre-warmed oxygenated media of the desired composition can be concomitantly delivered from the reservoirs to the superfusion chambers.

Synaptosomes are accomodated as very thin layers on microporous filters placed on glass filter supports. Synaptosome or slice super-fusion is provided by a multi-channel peristaltic pump and superfusate samples are directly collected into scintillation vials.
    FEATURES BENEFIT
    12 chambers are run simultaneously HIgh throughput for quick results
    Can be employed for synaptosomes and brain slices Allows for wide range of experiments
    Original design More than 300 papers published
    General
    Power Requirement 115 or 230 V, 50/60 Hz, 100 W max
    Operating Temperature 15° to 30° C
    Sound Level < 70 dB (A)
    Power Requirement 115 or 230 V, 50/60 Hz, 100 W max
    Physical
    Dimensions - Electronic unit cm 38 (w) x 30 (d) x 13 (h)
    Dimensions - Assembled bath cm 46 (w) x 28 (d) x 60 (h)
    Total Weight 34.00 Kg
    Shipping Weight 45.00 Kg approx
    Packing Dimensions

    No. 1 carton box 80x60x44 cm

    No. 1 carton box 66x50x63 cm

    Warranty 17400 is covered by 24-month warranty

    Neurotransmitter release is the major step of neuro-transmission. Abnormalities in neurotransmitter re-lease have been proposed to be involved in many pathological conditions.

    Therefore, understanding the physiological mecha-nisms of transmitter release and how the process can be modified by pathological states is essential to develop therapeutically useful pharmacological agents.

    Presynaptic nerve terminals are the sites where release specifically occurs; therefore superfusion of synaptosomes is best suited to explore presynaptic events.

    Superfused synaptosomes are the preparation of choice to study release-regulating presynaptic receptors and to explore the intimate mechanisms of neurotransmitter release.

    14900 14900 SUPERFUSION SYSTEM (Raiteri’s method), standard package, including
    14900-001 Electronic Unit
    14900-002 Superfusion Bath Complete Assem-bly, including upper & lower cham­bers, valves, set of tubes, etc.
    14900-004 Suction Pump
    14900-302 Instruction Manual
    14900-328 Set of Phials
    14900-338 Set of Filters
    14900-325 Phial Rack
    14900-302 Drain Collecting Pan

    {slider See Additional Bibliography}

    • A. Pittaluga et alia: "Effects of the neoclerodane Hardwickiic acid on the presynaptic opioid receptors which modulate noradrenaline and dopamine release in mouse central nervous system" Neurochemistry Intl. 62 (4): 354-359, 2013
    • S. Zucchini et alia: “Increased excitability in tat-transgenic mice: Role of tat in HIV-related neurological disorders” Neurobiology of Disease: available onlyne 2013
    • F. Giribaldi et alia: “Group I metabotropic glutamate autoreceptors induce abnormal glutamate exocytosis in a mouse model of amyotrophic lateral sclerosis” Neuropharmacology 66: 253-263, 2013
    • J. Marrocco et alia: “Anxiety-Like Behavior of Prenatally Stressed Rats Is Associated with a Selective Reduction of Glutamate Release in the Ventral Hippocampus” J. neuroscience 32 (48): 17143-17154, 2012
    • C. Romei et alia: “The GABAB receptor antagonists CGP35348 and CGP52432 inhibit glycine exocytosis: Study with GABAB1- and GABAB2-deficient mice” Pharmacological Res. 61: 547-552, 2010
    • M. Grilli et alia: “Specific Inhibitory Effect of Amyloid-β on Presynaptic Muscarinic Receptor Subtypes Modulating Neurotransmitter Release in the Rat Nucleus Accumbens” Neuroscience 167: 482-489, 2010
    • G. Bonanno et alia: “Release of [3H]D-aspartate induced by K+-stimulation is increased in the cervical spinal cord of the wobbler mouse: a model of motor neuron disease” Neurochemistry Intl. 55: 302-306, 2009
    • M. Grilli et alia: " Release-enhancing pre-synaptic muscarinic and nicotinic receptors co-exist and interact on dopaminergic nerve endings of rat nucleus accumbens" J. Neurochemistry 105 (6): 2205-2213, 2008

    Additional Papers:

    • A. Pittaluga et al.: “Human brain N-methyl-D-aspartate receptors regulating noradrenaline release are positively modulated by HIV-1 coat protein gp120AIDS 10: 463-468, 1996
    • M. Di Luca et al. “Increased presynaptic protein kinase C activity and glutamate release in rats with a prenatally induced hippocampal lesion” Eur. J. Neurosci. 9: 472-479, 1997
    • M.V. Clos et al. “D2 dopamine receptors and modulation of spontaneous acetylcholine (ACh) release from rat striatal synaptosomes” Br. J. Pharmacol. 122: 286-290, 1997
    • D. Crespi et al. “Carrier-dependent and Ca2+-dependent 5-HT and dopamine release induced by (+)amphetamine, 3,4-methylendioxymethamphetamine, p-chloroamphetamine and (+)fenfluramineBr. J. Pharmacol. 121: 1735-1743, 1997
    • E. Schlicker et al. “Effects of selective h5-HT1B (SB-216641) and h5-HT1D (BRL-15572) receptor ligands on guinea-pig and human 5-HT auto- and heteroreceptorsNaunyn-Schmiedeb. Arch. Pharmacol. 356: 321-327, 1997
    • G. Maura et al. “Glutamate release in human cerebral cortex and its modulation by 5-hydroxytryptamine acting at h 5-HT1D receptorsBr. J. Pharmacol. 123: 45-50, 1998
    • R. Sala et al. “Nerve growth factor and brain-derived neurotrophic factor increase neurotransmitter release in the rat visual cortexEur. J. Neurosci. 10: 2185-2191, 1998
    • M. L’Hirondel et al. “Lack of autoreceptor-mediated inhibitory control of dopamine release in striatal synaptosomes of D2 receptor-deficient miceBrain Research 792: 253-262, 1998

    In addition, more hundreds full papers using superfused synaptosomes have been published

    -  M. Raiteri, F. Angelini, G. Levi: “A simple apparatus for studying the release of neurotransmitters from synaptosomes“ Eur. J. Pharmacol. 25: 411-414, 1974