Pain and inflammation
Code: 35550

Thermal Gradient Ring (Zimmermann's method) Featured

The Thermal Gradient Ring is a novel device, which allows recording and analysis of Comprehensive Thermal Preference Phenotyping in Mice, according to Zimermann's method. 

In recent years the cellular and molecular mechanisms of temperature sensing and thermoregulation are subject of intensive research. Some researchers have evidenced the limits of the existing tests: to overcome current limitations we have designed a novel circular thermal gradient assay, for thermal preference phenotyping, based on the paper "Comprehensive thermal Preference Phenotyping in Mice using a Novel Automated Circular Gradient Assay", published by University Erlangen-Nuernberg (see Zimmmermann's paper). The new TGR (Thermal Gradient Ring) provides a high degree of freedom, i.e. thermal choice, and eliminates experimenter bias

The TGR is suitable to test neuropathic pain, and allows discerning exploratory behavior from thermal selection behavior!

The advantages brought about by the circular design are duplicate values, no border effects and no spatial cues, guaranteeing bias-free, reproducible data. The TGR is more sensitive than previous methods: gradient setup is superior to two plate choice design (reflects a more complex physiological environment, requires less time, less manpower and less mice).

The TGR consists of a circular running track, which provides a thermal gradient between the two extremes of a cold (15°C) and a hot zone (40°C) in which the mouse is free to move. The Thermal Insulated Ring-shaped Aluminium Runway has an ID of 45cm and 57cm OD.

A heater on one side of the ring, and cooler on the opposite side (based on the well tested technology employed in Ugo Basile Hot/Cold Plate NG), respectively heats and cools the extremes of the ring, creating a symmetric thermal gradient, controlled by 4 embedded thermocouples measuring the exact temperature gradient in real time.

Each side of the ring is divided into 12 zones, in which the temperature Δ is proportionally divided: for example, in the protocol described in the method paper, where the two preset temperatures are respectively 15°C and 40°C, each sector represents an increment of 2.27°C.

Gradient 1013x768

Recording and analysis of thermal preference behavior is accomplished by ANYmaze. Data output include:

  • Preference Temperature time course ± SD
  • Time lag to cover zones above a defined temperature (time course)
  • Zone histogram
     

Additional Info

    FEATURES

    BENEFIT

    New circular design, ID 45cm, OD 57cm

    Duplicate values, no border effects, no spatial cues

    Thermal Insulated Ring-shaped Aluminum Runway

    More sensitive than previous methods: bias-free, reproducible data

    Heater and cooler on opposite sides, to establish a symmetric gradient

    Gradient setup superior to two-plate choice design

    12 zones per side (specular), 40cm2 each

    Temperature Δ proportionally divided into 12 (in the method paper 15°C-40°C = 2.27°C per zone)

    4 thermocouples embedded in the thermal gradient ring

    The exact temperature gradient measured in real time

    CCD-camera and ANYmaze video-tracking software

    Behavior recorded automatically during 60 minutes

    General

     

    Operating temperature

    HEATER: from room temperature to 50°C

    COOLER: from 5°C 50°C  

    Controls

    Commands on the heater/cooler front panel

    Preset temperature Read-out

    On LED display on the heater/cooler front panel

    Detection

    Via ANYmaze software

    Temperature feedback

    Measured by 4 thermocouples and monitored by ANYmaze software in real time

    Sound

    Negligible

    Power 

    Universal input 85-264 VAC, 50-60Hz

    Physical

     

    Aluminum Runway

    ID 45cm, OD 57cm

    Circular Enclosures

    12cm high

    Dimensions

    65x90x32(h)cm

    Weight

    47Kg

    Shipping Weight

    59Kg

    Packing Dimensions

    82x71x57cm (wooden box)

    Warranty

    35550 is covered by a 24-month warranty

    "Currently available behavioral assays to quantify normal cold sensitivity, cold hypersensitivity and cold hyperalgesia in mice have betimes created conflicting results in the literature. Some only capture a limited spectrum of thermal experiences, others are prone to experimenter bias or are not sensitive enough to detect the contribution of ion channels to cold sensing because in mice smaller alterations in cold nociception do not manifest as frank behavioral changes. To overcome current limitations we have designed a novel device that is automated, provides a high degree of freedom, i.e. thermal choice, and eliminates experimenter bias. The device represents a thermal gradient assay designed as a circular running track. It allows discerning exploratory behavior from thermal selection behavior and provides increased accuracy by providing measured values in duplicate and by removing edge artifacts. Our custom-designed automated offline analysis by a blob detection algorithm is devoid of movement artifacts, removes light reflection artifacts and provides an internal quality control parameter which we validated. The assay delivers discrete information on a large range of parameters extracted from the occupancy of thermally defined zones such as preference temperature and skew of the distribution. We demonstrate that the assay allows increasingly accurate phenotyping of thermal sensitivity in transgenic mice by disclosing yet unrecognized details on the phenotypes of TRPM8-, TRPA1- and TRPM8/A1-deficient mice."

    from "Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay"

    35550

    Thermal Gradient Ring, complete assembly, including heater, cooler, circular runway and circular enclosure

     

    VIDEOTRACKING

    60000

    ANY-maze Software

    41700-040

    B/W USB Camera, including day&night 2.7-13.5mm varifocal lens, 5m USB cable and ceiling support

    Z. Winter, P. Gruschwitz, S. Eger, F. Touska and Katharina Zimmermann: "Cold Temperature Encoding by Cutaneous TRPA1 and TRPM8-Carrying Fibers in the Mouse" Front. Mol. Neurosci., 30 June 2017 , https://doi.org/10.3389/fnmol.2017.00209

    F. Touska Z. Winter, A. Mueller, V. Vlachova, J. Larsen and Katharina Zimmermann: "Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay" Journal Temperature, Volume 3, 2016 - Issue 1

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