Behaviour, Conditioning, Reward

46000 - Fear Conditioning System NG Featured

Fear Conditioning System Fear Conditioning System

The Ugo Basile Fear Conditioning System includes all the components to run experiments on mice or rats, according to the paradigms of contextual or cued fear conditioning

The detection of Freezing is automated and based on video analysis, by using the Any-Maze software (specific version for Fear Conditioning), which also controls the shocker and the sound generator.

The Ugo Basile Fear Conditioning systems are designed to run experiments of Pavlovian conditioning on mice or rats. Single-frequency sounds are delivered as neutral or conditioned, stimulus (CS). Experimental procedures which contemplate the sound as CS are usually referred to as Cued Fear Conditioning. Electric current from the grid floor is delivered as the naturally aversive, or unconditioned stimulus (US).

Tests are run in an animal cage with a grid floor, whose appearance can be easily altered by attaching patterned contexts on the walls and floor. This allows for the investigation of Contextual Fear Conditioning, in which the CS is not the sound but the visual appearance of the cage (i.e. the context) itself. In this case the animal associates the electric shock to the environment where it was received.

The detection of freezing is automated and based on video analysis. The shock, light and sound parameters are controlled by software (USB) or manually, via the new controller, based on touchscreen technology. The context kit provided with each cage alter the animal cage appearance, allowing for both Contextual and Cued procedures to be run with the same system. Our Fear Conditioning system works also in total darkness

The basic system includes the following components: 

  • Controller with touch-screen (with new application software NG)
  • Animal box(either rat or mouse) with electrified floor and Context Kit (3 floors, 9 walls)
  • Isolation Cubicle, dual (visible/I.R.) LED light, speaker and fan

The complete system also includes:      

  • Freezing-detection Software
  • USB videocamera

Preinstalled PC can be supplied as optional.

The 40500-001 Controller, incorporating a 12” touch-screen, encompasses all controls and incorporates a constant-current high precision 8-pole shocker. Up to 4 cages can be connected to the same controller.

The set-ups include Sound-proof isolation cubicles with dual light, speaker and fan: cubicles for special applications (for tethered animals, or with special lead sheets, to cut electromagnetic interference) are also available, see the specific web-page.

The FC set-up is part of UB conditioning-cage project; you buy a single touch-screen controller, and manage all UB cages. Ask for details!

Remote Control feature will make remote service and software upgrades extremely simple!

 

 

 

 

    FEATURES NEW ON THE 2014 VERSION!
    Part of the BEEHIVE SYSTEM The electronic unit for up to 4 animal cages!
    Automatic detection of Freezing Works also in total darkness
    Maximum Flexibility Configure your own Experimental Schedule, cued or conditioned
    Remote Control feature Makes remote service and software upgrades extremely simple!
    Specific versions for rats or mice Complete set-up, including sound-proof isolation cubicles
    Great Versatility The same controller can manage different conditioning tests. Ask for details!
    Touch Screen Controller
    LCD 12” with resistive touch screen
    CPU Module Port 2 USB Port 2.0; 1 Ethernet port 10/100Mb;1 DVI port for external monitor
    Peripheral Port 4 output for Sound, Shock and  light; 1 Power supply 12V-2A
    Expansion Bus         Connection 2 RJ11 connectors
    ANY-maze Port 1 USB port type B
    Digital Inputs 4
    Input Voltage TTL input 0-5Vdc opto-isolated
    General
    Shock Constant current
    Shock Intensity from 0.1 to 2.9 mA, in 0.1 mA steps
    Shock Duration Duration: from 0 to 99 seconds
    Visible Light 0-100%
    I.R. Light 0-100%
    Sound

    100Hz-18KHz, 0-100% (100%0100dB ±2dB)

    and white noise 0-100% (100%=72dB ±3dB)

    Power  80V/240Vac, 50/60 Hz
    Operating Temperature 10° to 40°C
    Sound Level Negligible (sound cue excluded)
    Pollution Degree ≤ 2
    Number of Cages Up to 4 (with expansion boxes)
    Physical
    Dimensions

    Cubicle            55(d)x60(w)x57(h)cm

    Controller        25(d)x33(w)x5.5(h)cm

    Mouse Cage   21(d)x24(w)x30(h)cm (internal: 17x17x25(h)cm) *

    Rat Cage        30(d)x34(w)x41.5(h)cm (internal: 26x26x30(h)cm)

    Weight 35Kg, for each cage set
    Shipping Weight 40Kg, for each cage set
    Packing Dimensions Wooden crate 82x71x57cm for each cage
    Warranty 48000 is covered by a 24-month warranty

    (*) A special Mouse Cage, dimensioned as the Rat model (internal 26x26x30cm) is also available as optional: the shock floor is spaced to accommodate mice - ask for model 46004

    Fear Conditioning is a quick and reliable method to assess memory in rodents.

    When rodents are exposed to fearful stimuli, they respond, among the others, with immobility behavior, also called “freezing”. All different Fear Conditioning procedures imply the measurement of the freezing response (i.e. the fraction of time spent immobile).

    A rodent can learn to fear a previous neutral stimulus if that has been associated with an instinctively aversive one (such as an electric shock), or, in other terms, after having been conditioned.

    After the conditioning has occurred, and the two stimuli have become associated, the animal is presented the neutral stimulus and, as a consequence, it will freeze. Freezing is commonly defined as complete immobility, apart from respiratory movements.

    The time spent freezing is indicative of undergone memory and learning processes, which are at the basis of the association between neutral and aversive stimuli.

    Fear Conditioning is a sensitive and quick test, which requires very little training (usually only one trial) to the animals. It is therefore a valuable tool for basic behavior research, as well as for high throughput and drug discovery studies. Moreover, the conditioning established during the Fear Conditioning procedure can be very strong (i.e. long-lasting), allowing for long term experiments.

     

      BASIC SYSTEMS  
    46102 Basic Rat Single-Cage Set-up, including controller with touch screen, application software, isolation cubicle with visible and I.R. light and speaker, cage. “Freezing” detection software and videocamera not included  
    46103 Basic Mouse Single-Cage Set-up, including controller with touch screen, application software, isolation cubicle with visible and I.R. light and speaker, cage. “Freezing” detection software and videocamera not included  
    46202 Basic Rat Two-Cage Set-up, including controller with touch screen, application software, isolation cubicle with visible and I.R. light and speaker, complete expansion-cubicle, two cages. Freezing” detection software and videocameras not included  
    46203 Basic Mouse Two-Cage Set-up, including controller with touch screen, application software, isolation cubicle with visible and I.R. light and speaker, complete expansion-cubicle, two cages. Freezing” detection software and videocameras not included  
    46402 Basic Rat 4-Cage Set-up, including controller with touch screen, application software, isolation cubicle with visible and I.R. light and speaker, 3 complete expansion-cubicles, 4 cages. Freezing” detection software and videocameras not included  
    46403 Basic Mouse 4-Cage Set-up, including controller with touch screen, application software, isolation cubicle with visible and I.R. light and speaker, 3 complete expansion-cubicles, 4 cages. Freezing” detection software and videocameras not included  
      INTERMEDIATE SYSTEMS, including videocamera without analysis software  
    46152-S Rat Single-Cage Set-up  
    46153-S Complete Mouse Single-Cage Set-up  
    46252-S Complete Rat Two-Cage Set-up  
    46253-S Complete Mouse Two-Cage Set-up  
    46452-S Complete Rat 4-Cage Set-up  
    46453-S Complete Mouse 4-Cage Set-up  
      COMPLETE SYSTEMS, including videocamera and sofware 60000-FC  
    46152 Complete Rat Single-Cage Set-up  
    46153 Complete Mouse Single-Cage Set-up  
    46252 Complete Rat Two-Cage Set-up  
    46253 Complete Mouse Two-Cage Set-up  
    46452 Complete Rat 4-Cage Set-up  
    46453 Complete Mouse 4-Cage Set-up  
      COMPONENTS  
    40500-001 Controller with 12" touch screen, controlling light, sound and shock parameters in up to 4 FC cages, including application software 46100-010
    46000-110 FC application software NG
    46002 Rat Cage for Fear Conditioning, with electrified grid floor and interchangeable floors and context kit
    46003 Mouse Cage for Fear Conditioning, with electrified grid floor and interchangeable floors and context kit
    46000-590 Isolation Cubicle for Fear Conditioning Cages, including dual visible/I.R. light, and speaker. Dimensions 50(w)x(40)d)x50(h) cm
    46000-580 Additional Isolation Cubicle for Fear Conditioning Cages, including dual visible/I.R. light, and speaker. Dimensions 50(w)x40(d)x50(h) cm. Slave-controller on board
    60000-FC Software ANY-maze for fear conditioning (freezing detection)
    47400-030 Complete BW Videocamera Fire-i, including standard and wide-angle lenses (without IR filter), power supply, FireWire 4.5m cable and 6to4 pin adaptor
    46102-002 Additional Rat Cage for Fear Conditioning System, including cage and expansion-cubicle with controller on-board, visible/I.R. light and speaker. Videocamera not included.
    46103-003 Additional Mouse Cage for Fear Conditioning System, including cage and expansion-cubicle with controller on-board, visible/I.R. light and speaker. Videocamera not included.

    See Additional Bibliography

    • D. Sierra-Mercado et alia: “Controlled Cortical Impact Be­fore or After Fear Conditioning does not Affect Fear Extinc­tion in MiceBrain Research 1606: 133-141, 2015
    • S. Yusufishaq et alia: "Post-Weaning Social Isolation im­pairs observational fear conditioning" Behav. Brain Res. 242 (1): 142-149, 2013
    • P.F. Fabene et alia: "Video-Based Analysis of Fear Conditioning: A Validation Test" Measuring Behavior 2012(2): 380, 2012
    • A. Sirri et alia: “Temporal Gene Expression Profile of the Hippocampus Following Trace Fear ConditioningBrain Re­search 1308 , 14-23, 2010

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